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Effects of the association of PO 4 /VitD on alveolar bone parameters and histomorphometric analysis in Hyp mice. (A) <t>Three-dimensional</t> <t>micro-CT</t> (mCT) presents mandibular alveolar bone. (B) PO 4 /VitD treatment does not improve alveolar bone volume fraction (BV/TV), BMD, and total mineral density (TMD) in Hyp mice. (C) Three-dimensional mCT reconstruction of the mandible showing the section plane used for histological and histomorphometric analyses in panels (E) and (F). (D) Three-dimensional mCT reconstruction of the mandible illustrating the orientation used for the sections shown in panels (E) and (F). (E) Von Kossa staining of undecalcified section of mice hemi-mandible. (F) Automated segmented-sections of mice mandibles to measure osteoid (green). (G) Histomorphometry analysis do not demonstrated a significant reduction of osteoid area/bone area (O.Ar/B.Ar) in alveolar bone after PO 4 /VitD treatment in Hyp mice.
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Effects of the association of PO 4 /VitD on alveolar bone parameters and histomorphometric analysis in Hyp mice. (A) Three-dimensional micro-CT (mCT) presents mandibular alveolar bone. (B) PO 4 /VitD treatment does not improve alveolar bone volume fraction (BV/TV), BMD, and total mineral density (TMD) in Hyp mice. (C) Three-dimensional mCT reconstruction of the mandible showing the section plane used for histological and histomorphometric analyses in panels (E) and (F). (D) Three-dimensional mCT reconstruction of the mandible illustrating the orientation used for the sections shown in panels (E) and (F). (E) Von Kossa staining of undecalcified section of mice hemi-mandible. (F) Automated segmented-sections of mice mandibles to measure osteoid (green). (G) Histomorphometry analysis do not demonstrated a significant reduction of osteoid area/bone area (O.Ar/B.Ar) in alveolar bone after PO 4 /VitD treatment in Hyp mice.

Journal: JBMR Plus

Article Title: Impact of oral phosphate supplements and active vitamin D treatment on dentoalveolar features of X-linked hypophosphatemia

doi: 10.1093/jbmrpl/ziag011

Figure Lengend Snippet: Effects of the association of PO 4 /VitD on alveolar bone parameters and histomorphometric analysis in Hyp mice. (A) Three-dimensional micro-CT (mCT) presents mandibular alveolar bone. (B) PO 4 /VitD treatment does not improve alveolar bone volume fraction (BV/TV), BMD, and total mineral density (TMD) in Hyp mice. (C) Three-dimensional mCT reconstruction of the mandible showing the section plane used for histological and histomorphometric analyses in panels (E) and (F). (D) Three-dimensional mCT reconstruction of the mandible illustrating the orientation used for the sections shown in panels (E) and (F). (E) Von Kossa staining of undecalcified section of mice hemi-mandible. (F) Automated segmented-sections of mice mandibles to measure osteoid (green). (G) Histomorphometry analysis do not demonstrated a significant reduction of osteoid area/bone area (O.Ar/B.Ar) in alveolar bone after PO 4 /VitD treatment in Hyp mice.

Article Snippet: WT and Hyp hemimandibles were scanned at 3 mo using an X-ray micro-CT device (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA, United States) at 90 kVp, 160 μA, an 180 s integration time, and a 10-μm voxel dimension.

Techniques: Micro-CT, Staining

Impact of the association of PO 4 /VitD on dentin and pulp chamber of Hyp mice. (A-C) Two-dimensional micro-CT (mCT) shows mandibular first molar (M1) enamel (E), dentin (DE), pulp chamber (PU), and alveolar bone (AB). Hyp mice present enlarged pulp chamber (asterisk), thin dentin, and altered alveolar bone (arrow) regardless of the presence or absence of treatment. (D-E) Treatment did not restore dentin/cementum volume or density. (F-H) Three-dimensional mCT presents first molar pulp. (I) Enlargement of pulp chambers was also not improved with a persistently enlarged chamber in treated Hyp mice.

Journal: JBMR Plus

Article Title: Impact of oral phosphate supplements and active vitamin D treatment on dentoalveolar features of X-linked hypophosphatemia

doi: 10.1093/jbmrpl/ziag011

Figure Lengend Snippet: Impact of the association of PO 4 /VitD on dentin and pulp chamber of Hyp mice. (A-C) Two-dimensional micro-CT (mCT) shows mandibular first molar (M1) enamel (E), dentin (DE), pulp chamber (PU), and alveolar bone (AB). Hyp mice present enlarged pulp chamber (asterisk), thin dentin, and altered alveolar bone (arrow) regardless of the presence or absence of treatment. (D-E) Treatment did not restore dentin/cementum volume or density. (F-H) Three-dimensional mCT presents first molar pulp. (I) Enlargement of pulp chambers was also not improved with a persistently enlarged chamber in treated Hyp mice.

Article Snippet: WT and Hyp hemimandibles were scanned at 3 mo using an X-ray micro-CT device (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA, United States) at 90 kVp, 160 μA, an 180 s integration time, and a 10-μm voxel dimension.

Techniques: Micro-CT